Method and kit for genotype detection of CYP3a53 sites

• Main Author: DU YUHE
• Format: Patent
• Language: Chinese; English
• Published: 19.06.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a method and a kit for genotype detection of CYP3a5*3 sites and relates to the field of molecular biology and medicine. Specific primers, probes and magnetic beads written intorelated report probes are designed for gene SNP sites related to risk of adverse reactions associated with dosage of tacrolimus and cyclosporine, and types of the SNP sites can be subjected to typingdetection. By means of the detection kit, the type of CYP3A5*3(G >A) in human whole blood samples can be detected sensitively and rapidly, reliable experimental evidence for application dosage adjustment of tacrolimus and cyclosporine can be provided for patients, adverse reactions are reduced, and clinical treatment is guided. 种对CYP3A5*3位点进行基因型检测的方法和试剂盒，本发明涉及分子生物学和医学领域，本发明针对使用他克莫司、环孢素剂量相关不良反应风险相关的基因SNP位点设计特异引物、探针以及写到相关报告探针的磁珠，可分型检测SNP位点的型别。本发明提供的检测试剂盒可以灵敏、快速低检测人全血样本中CYP3A5*3(G>A)型别，可为患者使用他克莫司、环孢素剂量调整提供可靠的实验证据，减少不良反应发生，指导临床治疗。