Method for detecting type 2 diabetic salivary protein marker

• Main Authors: SUN KEHUAN, FAN DAHUA, WU ZHENGZHI, CAO MEIQUN, HUANG FEIJUAN, YAO YONGCHAO, DING FENG
• Format: Patent
• Language: Chinese; English
• Published: 15.06.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting a type 2 diabetic salivary protein marker. The method comprises extracting a sample protein, acquiring the concentration of the extracted protein througha Bradford method, carrying out protein proteolysis, carrying out iTRAQ labeling, carrying out vacuum refrigeration and centrifugal drying, carrying out reversed-phase chromatography separation at high pH, carrying out ABI-5600-based protein analysis and mass spectrometry data analysis, in database detection, if the sample belongs to the sequenced organism, directly selecting the database of thesequenced organism, if the sample does not belong to the sequenced organism, selecting a database of a macroproteome with the largest correlation with the tested sample, and carrying out iTRAQ mass spectrometric analysis through the ABI-5600 mass spectrometer. The method can fast and reliably find a specific protein marker of diabetes through a proteome technical scheme and build a non-invasive, simple, rapid and practical