Genome editing vector, genome editing system constituted by genome editing vector and application

• Main Authors: LIU QIAN, LIN LIANGCAI, GAO JINGFANG, SUN WENLIANG, GAO RANRAN, TIAN CHAOGUANG, LI JINGEN
• Format: Patent
• Language: Chinese; English
• Published: 12.06.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a genome editing vector, a genome editing system constituted by the genome editing vector and an application, and in particular relates to a thermophilic fungi genome DNA editing vector, a genome editing system CRISPR/Cas9 constituted by the genome editing vector as well as an editing method an application. The genome editing vector comprises a promoter for promoting coding DNA transcription of sgRNA, wherein the promoter is an RNA polymerase III type U6 promoter. With the application of the genome editing system, genome editing efficiency of myceliophthora strains M.thermophile and M.heterothallica can be obviously improved, and simultaneous editing of multiple sites of myceliophthora genome can be implemented, so that polygenic mutant strains are obtained; the series of mutant strains can obviously improve production capacity of cellulase and can be applied to transformation of thermophilic fungi genetic engineering for high yield of the cellulase; the genomeediting system can also