Biosensor and method of forming probe on solid surface of biosensor

• Main Authors: TSAI RUNG-YWAN, CHEN WEN-YIH, TIAO GOLDEN
• Format: Patent
• Language: Chinese; English
• Published: 05.06.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a biosensor and a method for forming a probe on a solid surface of the biosensor. The method of forming the probe on the solid surface of the biosensor includes the following steps. A unit (a nucleotide or an amino acid) capped with a protecting group is covalently bonded on the solid surface of one of a plurality of sensor cells of the biosensor. At least one cycle of thefollowing steps is performed until a desired number of units is formed: irradiating the one of the plurality of sensor cells, so as to remove the protecting group of the unit; and binding a unit capped with the protecting group to the de-protected unit. The one of the plurality of sensor cells is irradiated to remove the protecting group, and then the probe is formed. 本发明公开了生物传感器和在生物传感器的固体表面上形成探针的方法。在生物传感器的固体表面上形成探针的方法包含以下步骤。将用保护基封端的单位共价结合于所述生物传感器的多个传感器单元之的固体表面上，其中所述单位是核苷酸或氨基酸。进行至少个循环，直到形成具有所需数目个单位的探针，个循环包括以下步骤：辐照所述多个传感器单元之，以去除单位的保护基；并且使以保护基封端的单位结合于脱保护的单位。辐照所述多个传感器单元之以去除保护基，从而形成探针。