Time resolution fluorescent immunochromatographic test strip and preparation method thereof

• Main Authors: CHEN QINGZHOU, GAO HONGMEI, LIU SONGYAN, ZHANG LEI, CHEN XIAOXIAO, PING XIATING, WANG ZHIHENG, XIAO MIAO, LI YUN, XUE ZHANYONG, HU YEJUN, ZHAO YILIANG, HU XIUZHI, SONG RUI, HE LINING, ZHAO XINGXIN, YANG JIE, SANG LIYA
• Format: Patent
• Language: Chinese; English
• Published: 01.06.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the field of the biological detection, and especially relates to a time resolution fluorescent immunochromatographic test strip and a preparation method thereof. The strip comprises a bottom plate, and a sample pad, a fluorescent microsphere pad, a reaction film, and a water-absorption pad closely connected with the bottom plate in order, wherein an anti-Malachite green monoclonal antibody-time resolution fluorescent microsphere coats on the fluorescent microsphere pad; a detection line and a quality control line are arranged on the reaction film; the Malachite green-bovine serum albumin conjugate coats on the detection line, and the goat-anti-mouse IgG secondary-antibody coats on the quality control line. The test strip disclosed by the invention can fast and quantitatively detect the Malachite green, the detection limit is low, and the stability is good; the operation is simpler and more convenient, and the test strip is especially suitable for the fast detection of a large number o