Method for continuously and stably producing high-purity raphanin

• Main Authors: ZHANG JIE, LUO NI, GUO YU, WEI HANG, SHAO XUEQING, HE JIALIN
• Format: Patent
• Language: Chinese; English
• Published: 01.06.2018
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of production of high-purity raphanin, and particularly relates to a method for continuously and stably producing the high-purity raphanin. According to the method, with crude myrosinase, crosslinked chitosan microspheres and radish seed meal as raw materials, the high-purity raphanin is produced through pretreatment, enzyme immobilization, reverse osmosis, centrifugal extraction, vacuum concentration and the like. The invention provides the method for continuously and stably producing the high-purity raphanin. The method has the characteristics ofsimple, stable, continuous and easily-amplified production technology, high product yield, low equipment investment cost, low production cost, high comprehensive resource utilization rate and the like. The high-purity raphanin prepared by the method provided by the invention has the characteristics of high product purity, stable performance and the like, and can be widely applied to medicines, health products, food, daily