Primer, probe and kit for detecting genotype of HPV (Human Papillomavirus) and usage method of kit

• Main Authors: DENG ZHONGPING, DAI LIZHONG, LIU BAOSHENG, LUO LIN, YANG BANGLIN, LI BO
• Format: Patent
• Language: Chinese; English
• Published: 22.05.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer, a probe, a kit and a usage method of the kit. A primer, a probe and a kit for detecting a genotype of HPV (Human Papillomavirus) and a usage method of the kit can beused for simultaneously detecting multiple HPV subgenotypes; through using a real-time fluorescence PCR (Polymerase Chain Reaction) amplification technique, the nucleic-acid extraction only needs to be carried out once; a PCR detection reaction also needs to be carried out once in each group; the usage method of the kit is quick, simple and convenient; moreover, the specificity is good; other HPVsubgenotypes or other microbes and the like cannot be detected out; therefore, a false positive problem difficultly appears; moreover, the sensitivity is high, and can reach 400copies/ml; a detectionrange is 400copies/ml to 4.00E+09copies/ml, and a detection range is wide. 本发明公开了种引物和探针、试剂盒及试剂盒的使用方法。该HPV分型检测用的引物和探针、试剂盒和试剂盒的使用方法可以同时检测多种HPV亚型，通过使用实时荧光PCR扩增技术，只需进行次核酸提取，各组也只需要进行次PCR检测反应即可，该试剂盒的使用方法快速简便，并且特异性好，对其他HPV亚型或其他微生物等不会检