Bovine monocyte chemoattractant protein-1 hybridoma cell strain, a monoclonal antibody secreted by same and application

• Main Authors: XIA AIHONG, ZHU ZHAOCHENG, YIN YUELAN, JIAO XIN'AN, CHEN XIANG, SUN LIN, LI XIN, LIU ZE, MENG CHUANG, PAN ZHIMING, XU ZHENGZHONG
• Format: Patent
• Language: Chinese; English
• Published: 18.05.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a hybridoma cell strain 4G9 secreting a bovine monocyte chemoattractant protein 1 (MCP-1) resisting monoclonal antibody as well as a monoclonal antibody MAb 4G9 secreted by same. The monoclonal antibody MAb 4G9 has the advantages of high titer, good specificity and high affinity with natural bovine MCP-1 antigen, and a Western-Blot detection kit, a direct immunofluorescence(DFA) detection kit and a flow cytometry (FCM) detection kit established on the monoclonal antibody MAb 4G9 have good sensitivity and specificity and can be used for detecting recombinant bovine MCP-1, detecting in vitro tissues, researching and identifying the epitope, performing the import and export inspection and quarantine, and performing the non-diagnosis research such as epidemiologic studyand the like. 本发明提供了分泌抗牛单核细胞趋化蛋白1(MCP-1)单克隆抗体的杂交瘤细胞株4G9，以及其分泌的单克隆抗体MAb 4G9。该单克隆抗体MAb 4G9具有效价高、特异性好、与天然牛MCP-1抗原亲和力强的优势，基于此建立的Western-Blot检测试剂盒、直接免疫荧光(DFA)检测试剂盒和流式细胞术(FCM)检测试剂盒，具有良好的灵敏度和特异性，可以用作重组牛MCP-1的检测、对离体组织进行检测、表位鉴定研究、进出口检