Marine-derived superoxide dismutase, as well as coding gene and application thereof

• Main Authors: QUAN LIFANG, ZHANG SHAN, DONG ZHIYANG, DONG LIANG, HE YONGZHI
• Format: Patent
• Language: Chinese; English
• Published: 11.05.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a marine-derived superoxide dismutase, as well as a coding gene and application thereof. The protein is as shown in (a1) or (a2), wherein (a1) is protein prepared from amino acid sequences as shown in the sequence I in a sequence table; and (a2) is proteins which are derived from the amino acid sequence of the sequence 1, are substituted and/or lost and/or added with one ormore amino acid residues and have the same function. According to the marine-derived superoxide dismutase, meta-genome of Southwest Indian Ocean sediment samples is sequenced, and Io-SOD1 is discovered and obtained, the expression of the gene in colibacillus is successfully realized; the expression method is simple and feasible; the expression product can be easily purified, has good stability, and has the specific activity reaching 1315U/mg; and the dismutase has excellent stability. The Io-SOD1 has broad industrial application prospect. 本发明公开了种海洋来源超氧化物歧化酶及其编码基因与应用。本发明提供了种蛋白质，是如下(a1)或(a2)：(a1)由序列表中序列1所示的氨基酸序列组成的蛋白质；