Method and kit for detecting SNP locus genotypes having adverse reactions to CTX drugs

• Main Author: DU YUHE
• Format: Patent
• Language: Chinese; English
• Published: 08.05.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a method and kit for detecting SNP locus genotypes having adverse reactions to CTX drugs. The invention relates to the fields of molecular biology and medicine; according to the kit provided by the invention, aiming at SNP loci with cyclophosphamide adverse reaction risks and in human genomic DNA, namely a GSTP1 gene rs1695, specific primers and probes are designed, a hybridization reaction with MagPlex-TAG magnetic beads with corresponding report genes is performed, and detection is performed on a luminex 200 instrument through chromogenic reaction; a base type of eachSNP locus is judged through reading and interpretation of signal values. The kit has the beneficial effects that the specific primers, probes and report gene probe sequences are designed by using theSNP loci related to the adverse reaction risks of cyclophosphamide used by patients with systemic lupus erythematosus and other diseases, and the types of the SNP loci can be detected in a typing manner. The detection kit pro