Naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method
The invention discloses a naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method. The naphthalimide compound with a structure of a formula (I) is applied tothe Ag+ detection in the HL-60 cell, and has high Ag+ sensitivity and response value; when it is used...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
17.04.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method. The naphthalimide compound with a structure of a formula (I) is applied tothe Ag+ detection in the HL-60 cell, and has high Ag+ sensitivity and response value; when it is used as the fluorescence probe, the fluorescence probe has obvious response for Ag+; when Ag+ with different concentrations is detected, the fluorescence probe shows good linear relationship; the fluorescence probe also has good selectivity of Ag+ in the HL-60 cell; the HL-60 suspending cell dyed withnaphthalimide compound is activated by blue light; through observation in a microscope, the cell emits green fluorescence, and the detection method is reliable.
本发明公开种能识别HL-60细胞内Ag的荧光探针萘酰亚胺类化合物及其制备方法,本发明提供的具有式(Ⅰ)结构的萘酰亚胺类化合物应用于HL-60细胞内Ag检测,对Ag灵敏度高,响应值高;其作为荧光探针使用时,对Ag具有明显的响应;对于不同浓度的Ag进行检测时,呈现出良好的线性关系;且对于在HL-60细胞内对Ag有很好的选择性,染有萘酰亚胺类化合物的HL-60悬浮细胞经蓝光激发后,在显微镜中观察,细胞发出绿色荧光,检测方法可靠;。 |
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Bibliography: | Application Number: CN201711281620 |