Naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method

• Main Authors: XU JIANHUA, KE FANG, LIN CHEN, WU LIXIAN, LIN SHANSHAN, XU SHUQING
• Format: Patent
• Language: Chinese; English
• Published: 17.04.2018
• Subjects: ADHESIVES; CHEMISTRY; DYES; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; TESTING

The invention discloses a naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method. The naphthalimide compound with a structure of a formula (I) is applied tothe Ag+ detection in the HL-60 cell, and has high Ag+ sensitivity and response value; when it is used as the fluorescence probe, the fluorescence probe has obvious response for Ag+; when Ag+ with different concentrations is detected, the fluorescence probe shows good linear relationship; the fluorescence probe also has good selectivity of Ag+ in the HL-60 cell; the HL-60 suspending cell dyed withnaphthalimide compound is activated by blue light; through observation in a microscope, the cell emits green fluorescence, and the detection method is reliable. 本发明公开种能识别HL-60细胞内Ag的荧光探针萘酰亚胺类化合物及其制备方法，本发明提供的具有式(Ⅰ)结构的萘酰亚胺类化合物应用于HL-60细胞内Ag检测，对Ag灵敏度高，响应值高；其作为荧光探针使用时，对Ag具有明显的响应；对于不同浓度的Ag进行检测时，呈现出良好的线性关系；且对于在HL-60细胞内对Ag有很好的选择性，染有萘酰亚胺类化合物的HL-60悬浮细胞经蓝光激发后，在显微镜中观察，细胞发出绿色荧光，检测方法可靠；。