Method for detecting chronic nephritis biomarkers based on salivary proteomics

• Main Authors: SUN KEHUAN, FAN DAHUA, WU ZHENGZHI, CAO MEIQUN, YAO YONGCHAO, HUANG FEIJUAN, DING FENG
• Format: Patent
• Language: Chinese; English
• Published: 03.04.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting chronic nephritis biomarkers based on salivary proteomics. The method comprises the following specific steps: sample protein extraction; determination ofthe concentration of extracted protein by using a Brad ford method; proteolysis; iTRAQ marking; vacuum refrigerated centrifugation drying; reversed phase chromatography under a high pH condition; protein analysis by using ABI-5600; mass spectral data analysis, namely when selecting a database, in case of an sequenced organism, a database for this species is directly selected, and in case of a non-sequenced organism, a database for a large-range proteome most relevant to a sample to be tested is selected; iTRAQ mass spectrometric analysis by using an ABI-5600 mass spectrometer. The method canbe used for finding out specific protein markers in chronic nephritis by using quick and reliable proteomics technology scheme, so as to establish a non-invasive, easy, convenient, fast, and practicaldetection assessment for e