Sucrose phosphorylase mutant and application thereof in production of glycerol glucoside

• Main Authors: SHI LUQIU, SU GUIZHEN, WANG CHANGGUO, XUE HONGYU
• Format: Patent
• Language: Chinese; English
• Published: 30.03.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention aims to provide a sucrose phosphorylase mutant and application thereof in the production of glycerol glucoside. The sucrose phosphorylase mutant which is protein is obtained by replacingthe 341st-site amino acid residue, which is leucine, of sucrose phosphorylase with other amino acid residues. The protein can be BaSP/L341W protein obtained by replacing the 341st-site amino acid residue, which is the leucine, of the sucrose phosphorylase with tryptophan. The sucrose phosphorylase mutant has the advantages that by the primer point mutation of the 341st-site amino acid residue ofthe wild sucrose phosphorylase derived from bifidobacterium adolescentis, the specificity of 2-glycerol glucoside produced by using the sucrose phosphorylase mutant, sucrose and glycerin as the raw materials, and the yield of the byproduct 1-glycerol glucoside is lowered; the sucrose phosphorylase mutant has an important application value in the field of the production of the 2-glycerol glucoside. 本发明公开了本发明的目的是提供种蔗糖磷酸化酶突变体