Method for detecting enzymatic activity of biotin enzyme

• Main Authors: XU PENGQI, RONG ENGUANG, HAO SHUJING
• Format: Patent
• Language: Chinese; English
• Published: 20.03.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for detecting enzymatic activity of biotin enzyme. A reagent 1 adopted by the method comprises a system formed by a buffer solution and an enzymatic activity stabilizer, the pH value is 4-8; a reagent 2 adopted by the method comprises a substrate solution, the component of the substrate solution is N-D-biotin-7-amino-4-methyl coumarin, and the concentration is 0.05mmol/L to 5mmol/L; a reagent 3 adopted by the method is a stop buffer; and a reagent 4 adopted by the method is a standard substance. According to the method for detecting enzymatic activity of biotinenzyme, N-D-biotin-7-amino-4-methyl coumarin is used as a substrate, detection can be directly performed without performing derivatization reaction after reaction is terminated, the operation is simple, relatively high detection sensitivity and wider detection linearity are achieved, and a sample can be directly used for enzymatic activity detection without earlier stage processing of any form, thus being convenient and q