Telomere probe and preparation method and application thereof

• Main Authors: CUI YIPING, ZONG SHENFEI, WANG LE, WANG ZHUYUAN, CHEN CHEN
• Format: Patent
• Language: Chinese; English
• Published: 23.02.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a telomere probe and a preparation method and application thereof. According to the telomere probe, a single stranded DNA is coupled onto the surface of the CdSSe/ZnS quantum dots, the telomere probe can be combined with the cell telomere by in-situ hybridization technique, and the telomere hybridized with the telomere probe is imaged by a single molecular positioning technique to realize the detection of the telomere. The telomere probe realizes the specific marking of the telomere, has higher resolution due to the adoption of the single molecule positioning microscopeimaging, and overcomes the limitation by the optical diffraction limit of the fluorescence microscopic technique. The telomere probe and the preparation method and the application thereof can be usedfor further study of the telomere structure. 本发明公开了种端粒探针及其制备方法和应用，该端粒探针为在CdSSe/ZnS量子点表面偶联了单链DNA，该端粒探针可以通过原位杂交技术结合到细胞端粒，用单分子定位技术对杂交了端粒探针的端粒进行成像，实现对端粒的检测。本发明实现了端粒的特异性标记，利用单分子定位显微镜成像，分辨率更高，克服了荧光显微技术的受光学衍射极限限制的缺点，利用本发明可以实现对端粒结构的进