Heparin sodium detection through proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA

• Main Authors: WENG SHAOHUANG, LI FENGLAN, GUO RUBIN, LIN XINHUA, ZHAO DONG
• Format: Patent
• Language: Chinese; English
• Published: 16.02.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses heparin sodium detection through a proportional fluorescence method based on carbon-quantum-dot-quenched fluorescence-labeled DNA. Heparin sodium can recover the fluorescence of carbon-quantum-dot-quenched FAM-labeled DNA, with the concentration rise of heparin sodium, recovery of FAM is improved, and the original fluorescence intensity of carbon quantum dots is kept stable, thereby establishing a new ratio-type fluorescence method for heparin sodium detection. The heparin sodium detection method is simple in steps, and heparin sodium and DNA fluorescence recovery showgood linear correlation. The linear correlation is low and can reach 9.04 ng/mL. In fact, the recovery of heparin sodium in a rabbit serum sample is in a range of 95.47-111.95%. A fitted curve for simulating detection of heparin sodium in serum is close to the standard curve. Therefore, a serum sample doesn't interfere in a detection result. Compared with other heparin sodium detection methods, the method is excellent in