PET hydrolase mutant with high catalytic activity

• Main Authors: ZHENG XUELIAN, ZOU JIAJIA, ZHANG YONG, LIU BINGLIN, LI SIYANG, TANG LIXIA
• Format: Patent
• Language: Chinese; English
• Published: 09.02.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of protein engineering, and specifically relates to a PET hydrolase mutant with high catalytic activity. The technical problem to be solved by the invention is thatthe activity of PET hydrolase (PETase) derived from Ideonella sakaiensis 201-F6 bacterial strains at present is still unsatisfactory. The technical scheme of the technical problem solved by the invention is to provide the PET hydrolase mutant. Five mutation hot spots are obtained through a large number of researches of PET hydrolase (PETase); fourteen mutants are constructed by adopting a site-specific mutagenesis technology, and finally the activity of ETase enzyme of two screened mutants are improved compared with that of wild type Ec_PETase, and the PET hydrolase mutant has preferable application prospects. 本发明属于蛋白工程领域，具体涉及种PET水解酶突变体。本发明要解决的技术问题是目前来源于Ideonella sakaiensis 201-F6菌株中的PET水解酶(PETase)的活性还不理想。本发明解决技术问题的技术方案是提供了PET水解酶突变体。本发明通过对PET水解酶(PETase)的大量研究，共获得5个突变热点；应用定点突变技术构建了14个突变体，最终筛选出2个突变体ETase酶的活性较野生型Ec_PE