Pseudomonas putida with transformed red fluorescent protein and application of pseudomonas putida

• Main Authors: ZHAO HEJUAN, WEI QISHUN, WANG LIN, ZHOU YING, WEN WEIMING, SHAO HEPING
• Format: Patent
• Language: Chinese; English
• Published: 23.01.2018
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a pseudomonas putida with transformed red fluorescent protein and an application of the pseudomonas putida to promotion of agaricus bisporus growth. The pseudomonas putida is collected in the China General Microbiological Culture Collection Center on June 29, 2017, and the collection number of the pseudomonas putida is CGMCC NO. 14367. The red fluorescent protein is transformed into a thallus by means of genetic engineering, the colonization condition of the pseudomonas putida in an agaricus bisporus soil covering layer can be detected, 10 -10 cfu/g covering soil isapplied to the soil covering layer, agaricus bisporus kinking can be effectively promoted, the yield and the quality of agaricus are improved, and the pseudomonas putida has the development prospectof agaricus bio-fertilizers. 本发明公开了株转化了红色荧光蛋白的恶臭假单胞菌（）及对双孢蘑菇生长促进作用，该菌株已于2017年6月29日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为：CGMCC NO.14367；本发明利用基因工程手段在菌体中转入了红色荧光蛋白，有利于检测菌株在双孢蘑菇覆土层中的定殖情况，在覆土层中施用10-10cfu/g覆土，能有效促进双孢蘑菇扭结，提高蘑菇的产量和品质，具有蘑菇生物肥料的开发前