Two-dimension nuclear magnetic resonance detection method used for water-soluble metabolites of in-vitro cells

• Main Authors: LI HONGXIA, LI FUYAN, WANG XUAN, SHI WENQI, WANG XIAO, ZENG QINGSHI, XU YANJIE, ZHANG ZHAOTAO, GAO WENJING
• Format: Patent
• Language: Chinese; English
• Published: 19.01.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a two-dimension nuclear magnetic resonance detection method used for water-soluble metabolites of in-vitro cells, which particularly includes the steps of: 1) washing culturedcells with a frozen phosphate buffer solution, performing centrifugation, and collecting cell deposits; 2) adding a phosphate buffer solution containing sodium silicate to the cell deposits to extractthe water-soluble metabolites of the cell line; and 3) performing the two-dimension NMR detection to the water-soluble metabolite extract in the step 2) to obtain relative contents of the water-soluble metabolites. In the method, steps of cell pretreatment, extraction of the water-soluble metabolites and NMR detection are reasonably optimized, so that extraction and detection efficiency of the water-soluble metabolites are increased. The method achieves accurate measurement of change on levels of the water-soluble metabolites of the in-vitro cells. 本发明公开了种用于离体细胞水溶性代谢物的二维核磁共振检测方法。方法具体为：1)将培养好的细胞用冰冻的磷酸盐缓冲液冲洗离心后收集细胞沉淀；2)