Kit for real-time PCR (Polymerase Chain Reaction) detection of pigeon TTV (Torque teno virus) and novel pigeon adenovirus

• Main Authors: CHENG LONGFEI, HUANG YU, CHEN HONGMEI, SHI SHAOHUA, FU QIULING, CHEN CUITENG, FU GUANGHUA, LIU RONGCHANG, WAN CHUNHE
• Format: Patent
• Language: Chinese; English
• Published: 19.01.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a kit for real-time PCR (Polymerase Chain Reaction) detection of pigeon TTV (Torque teno virus) and novel pigeon adenovirus. The kit comprises following two pairs of primers andtwo probes, the specific sequences thereof are as shown in SEQ ID NO.1-6. According to the invention, the kit can establish a detection method for identifying and diagnosing pigeon TTV and novel pigeon adenovirus in pigeon groups, the operation procedures are simplified and the cost is saved. 本发明提供种鸽TTV和鸽新型腺病毒双重Real time PCR检测试剂盒，所述试剂盒包括以下2对引物和2条探针，具体序列如SEQ ID NO.1-6所示。本发明通过所述试剂盒建立能够同时对鸽群中鸽TTV和鸽新型腺病毒鉴别诊断的检测方法，操作程序简化、节约成本。