Kappa-carrageenase, gene encoding same and application of kappa-Carrageenase

• Main Authors: XIAO ANFENG, XIAO QIONG, CAI HUINONG, JIANG ZEDONG, HONG QINGLIN, NI HUI, JI HAIFENG, ZHONG XIAOTING, LI JIAJIA, CHEN CHUIYE
• Format: Patent
• Language: Chinese; English
• Published: 19.01.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses kappa-carrageenase, a gene encoding the same and an application of kappa-carrageenase. The cloned kappa-carrageenase precursor gene car 30 of P.carrageenovora ASY5 is transformed into E. coli, a recombinant bacterium producing recombinant kappa-carrageenase is obtained, and the suitable temperature and pH of the recombinant kappa-carrageenase are 45 DEG C and 6.5; after heat preservation at 40 DEG C for 30 min, the remnant enzyme activity is 80.0% or higher, the recombinant kappa-carrageenase is treated for 60 min in a buffer solution with pH being 6.5-7.5, and the enzyme activity can still remain 50.0% or higher. The recombinant vector containing the kappa-carrageenase gene car 30 is constructed, heterologous expression is realized, and a good foundation is provided for industrialized production and application of the enzyme. The expressed kappa-carrageenase has a better degradation effect on kappa-carrageenan, kappa-carrageenan is degraded into disaccharide, tetrasccharide and the li