YEAST STRAIN HAVING IMPROVED CAPABILITY FOR FERMENTING XYLOSE IN THE PRESENCE OF ACETIC ACID

• Main Authors: DESFOUGERES THOMAS, TECHEL JENNIFER, PIGNEDE GEORGES, BAVOUZET JEAN-MICHEL
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid,in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form. 本发明涉及种筛选酵母菌株的方法，所述酵母菌株在非解离形式的有机酸，优选地是乙酸的存在下具有提高的发酵戊糖，优选地是木糖的能力，其中至少株能够发酵所述戊糖的酵母菌株在以下两种培养基中连续培养：第生长培养基，其包含作为唯碳源的所述戊糖和非解离形式的所述有机酸；第二生长培养基，其包含作为唯碳源的另碳源，优选地为葡萄糖，不含非解离形式的所述有机酸，在浓度升高的非离解形式的有机酸存在下，至少在所述两种生长培养基中连续培养，重复至少两次。