Method for accurately identifying genotypes based on high-throughput sequencing, and application of method

• Main Authors: WU QIANG, WANG LEYANG, HUANG HAIYAN, BAI YUMENG
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a method for accurately identifying genotypes. The method is based on high-throughput sequencing and comprises the following steps: carrying out high-throughput sequencing on a gene fragment obtained by amplifying so as to obtain a nucleotide sequence set; treating the nucleotide sequence set for excluding interfering sequences so as to obtain an effective nucleotide sequence set; carrying out repetitive sequence analysis on the effective nucleotide sequence set; carrying out statistic analysis on the effective nucleotide sequence set, and outputting a result. The method can be used for effectively and accurately detecting the genotypes of all chromosomes in mutant cells or tissues, can use and automatically remove the nonspecific sequences which are brought in due to sequencing, and summarizes the percentage of each mutation sequence, thus having very practical business value. 本发明公开了种精确鉴定基因型的方法，其基于高通量测序，包括以下步骤：对扩增获得的基因片段进行高通量测序，获得核苷酸序列集合；对核苷酸序列集合进行处理，排除干扰序列，获得有效核苷酸序列集合；对有效核苷酸序列集合进行重复序