Construction method for MACF1 site-specific transgenic mice model

• Main Authors: LI DIJIE, QIAN AIRONG, LI SIYU, ZHAO FAN, CHEN ZHIHAO, ZHANG RU, WANG PAI, QIU WUXIA, CHEN LEI, SU PEIHONG, YIN CHONG, YANG CHAOFEI, ZHANG YAN, MA XIAOLI, HU LIFANG
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a construction method for an MACF1 site-specific transgenic mice model. The construction method is adopted for solving the technical problem of poor practicability of the present construction method for the transgenic mice model. According to the technical scheme, a carrier for an exogenous MACF1 gene is firstly constructed and then is site-specifically transferred to a mice Rosa26 locus, so as to acquire the MACF1 site-specific transgenic mice model. The descendant mice of the mice model, which are not mated with Cre mice, will not excessively express MACF1, and MACF1 over-expressed mice with different tissue specificities can be acquired in the manner of mating with different Cre mice. The mice model has significant application values in researching MACF1 function and the pathogenesis of the MACF1 related diseases and screening the candidate substance, drug target, and the like, for improving or treating the diseases and has high practicability. 本发明公开了种MACF1定点转基因小鼠模型构建方法，用于解决现有转基因小鼠模