Cold-water fish probiotic Lactococcus lactis strain and uses thereof

• Main Authors: CUI HONGYU, LU TONGYAN, WANG YUNFENG, WANG XIAOMEI
• Format: Patent
• Language: Chinese; English
• Published: 01.12.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The present invention discloses a cold-water fish probiotic Lactococcus lactis strain and uses thereof. According to the present invention, a strain HZC32 capable of providing functions of antivirus, immunoloregulation and immune enhancing for cold-water fishes is isolated and identified from the intestinal mucosa of semi-wild juvenile rainbow trout in Benxi Mountainous area of Liaoning Province; according to the morphological characteristic observation of the bacterial colony, the 16S rDNA base sequence determination, and the homology analysis, the identification results of the strain comprise that HZC32 belong to Lactococcus lactis, and the preservation number is CGMCC No. 10705; the in vitro cell experiment results show that the in vitro anti-IHNV-proliferation in EPC cells can be provided after the co-culture of the Lactococcus lactis HZC32 strain and the virus-infected EPC cells; the animal experiment results show that the Lactococcus lactis HZC32 strain can provide immunoloregulation and immune enhancin