Methods and systems for biological instrument calibration

• Main Authors: CHU YONG, WOO DAVID, FREUDENTHAL JACOB, WESSEL THOMAS, MARKS JEFFREY
• Format: Patent
• Language: Chinese; English
• Published: 28.11.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

In one exemplary embodiment, a method (100) for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration (102) to determine reaction site positions in an image. The method further includes performing a pure dye calibration (108) to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration (110) to determine a filter normalization factor. The method includes performing an RNase P validation (112) to validate the instrument is capable of distinguishing between two different quantities of sample. 在个示例性实施例中，提供了种用于校准仪器的方法(100)。所述仪器包括个能够对多个反应位点荧光发射进行成像的光学系统。该方法包括执行感兴趣区域(ROI)校准(102)以确定图像中的反应位点位置。该方法还包括执行纯染料校准(108)以通过比较荧光染料的原始谱图与荧光染料的纯谱图校准数据来确定各个反