Porcine circovirus purifying method

The invention discloses a porcine circovirus purifying method which includes the steps: first, centrifuging porcine circovirus cell culture fluids, removing precipitates and collecting supernate; second, transversely cutting and concentrating the supernate by a transverse cutting concentration filte...

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Main Authors WU DAXING, ZHANG SHUQIONG, LU JING, XU JIAHUA, LIAO SHICHANG, CHEN RUI'AI
Format Patent
LanguageChinese
English
Published 24.11.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a porcine circovirus purifying method which includes the steps: first, centrifuging porcine circovirus cell culture fluids, removing precipitates and collecting supernate; second, transversely cutting and concentrating the supernate by a transverse cutting concentration filter membrane; third, adding pure water into concentrated solution; fourth, repeating the second step and the third step for 3-5 times; fifth, performing weak anion exchange chromatography on the concentrated solution, performing linear elution and then collecting a first elution peak to obtain porcine circovirus fluids; sixth, concentrating the porcine circovirus fluids by an ultra-filter membrane; seventh, performing molecular sieve chromatography separation on the concentrated solution obtained in the sixth step by a gel chromatography medium, and collecting a first chromatographic peak to obtain porcine circovirus pure fluids. The purifying method has the advantages of simplicity in operation, large purifying amou
Bibliography:Application Number: CN201710787727