用于基因组目标区域捕获测序的杂交富集溶液及试剂盒

• Format: Patent
• Language: Chinese
• Published: 23.03.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

本发明提供了一种用于基因组目标区域捕获测序的杂交富集溶液和试剂盒，所述杂交富集溶液由SSPE、EDTA、Denharts溶液及SDS组成。本发明公开的改进的杂交富集溶液配方简单，成本较低且容易保存，能够特异性的提取目标DNA片段，适合第二代测序中的捕获杂交富集基因组目标区域的操作。 The invention provides a hybridization enrichment solution for genome targeted region capture sequencing and a kit. The hybridization enrichment solution is prepared from SSPE, EDTA, a Denharts solution and SDS. The improved hybridization enrichment solution disclosed by the invention has the advantages that the formula is simple; the cost is low; the storage is easy; target DNA fragments can be specifically extracted; and the improved hybridization enrichment solution is suitable for the operation of capturing a hybridization enrichment genome targeted region in the second generation sequencing.