Peanut leaf tissue grinding method and peanut leaf tissue grinding device for extracting DNA

The invention discloses a peanut leaf tissue grinding method and a peanut leaf tissue grinding device for extracting a DNA and belongs to the technical field of biotechnology. The tip of a pipette head is fired into a round ball through heating of an alcohol lamp; the pipette head of which the tip i...

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Bibliographic Details
Main Authors ZHENG YIXIONG, LI CHUNJUAN, YAN CAIXIA, WANG JUAN, SHAN SHIHUA, ZHAO XIAOBO
Format Patent
LanguageChinese
English
Published 17.10.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a peanut leaf tissue grinding method and a peanut leaf tissue grinding device for extracting a DNA and belongs to the technical field of biotechnology. The tip of a pipette head is fired into a round ball through heating of an alcohol lamp; the pipette head of which the tip is fired into the round ball is matched with an existing centrifuge tube, and the peanut leaf tissues cooled through liquid nitrogen are mashed to achieve direct extraction of the DNA in the mashed peanut leaf tissues in the centrifuge tube. Compared with mashing of the peanut leaf tissues achieved by adopting a mortar and pestle coordination mode in the prior art, waste of the peanut leaf tissues in the grinding process is reduced and the peanut leaf tissue grinding method is more suitable for extraction of the DNA in the peanut leaf tissues when the peanut leaf tissues are relatively few; meanwhile, compared with an automatic sample grinding instrument in the prior art, the extraction cost of the DNA in peanut lea
Bibliography:Application Number: CN201710686110