Gene microfluidic chip for detecting 26 animal-derived components at same time and application thereof

• Main Authors: GUO MIAO, LIU JIAN, YANG HONGLIAN, LI HEJING, YANG XINTING, HU ZHIKAI, ZHAO LINNA, HUANG HUA, HAN YUEBEI, WANG DAN, XUE CHENYU
• Format: Patent
• Language: Chinese; English
• Published: 13.10.2017
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The invention provides a gene microfluidic chip for detecting 26 animal-derived components at the same time and application thereof, and belongs to the technical field of animal food detection. 32 combinations each of which consists of a pairs of primers and a probe are fixed to the gene microfluidic chip, and the nucleotide sequences are shown as SEQ ID NO.1-96; fluorescence quantitative PCR detection is performed on a sample to be detected, and according to a Ct value, whether the sample to be detected contains any of the 26 animal-derived components is judged. By a detection method provided by the invention, rapid identification detection of the 26 animal-derived components can be completed within 3 hours; the detection method has the advantages of accurate detection, high sensitivity, strong specificity, simpleness, convenience and rapidness; the lowest detection limit is 1pg; requirements on large-batch and rapid identification detection of the 26 animal-derived components can be met; adulteration of mea