Method for application of plant growth regulator in streptomycete microbial inoculum preparation

• Main Authors: GAO XIAOSHA, CHANG DAYONG, CHANG XIAOFEI
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATERTREATMENT OR WASTE MANAGEMENT; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERTILISERS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; MANUFACTURE THEREOF; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The invention relates to a method for application of a plant growth regulator in streptomycete microbial inoculum preparation, and the method comprises the following steps: 1) strain shaking culture; 2) seed liquid preparation; 3) fermentation liquid preparation; and 4) microbial inoculum fixation. The industrial production of a cellulase producing streptomycete microbial inoculum can be realized by a three stage fermentation method. Especially in the process of seed tank and fermentation tank culture, the plant growth regulator such as triacontanol, sodium nitrophenolate and diethylaminoethyl hexanoate is added to significantly shorten the seed growth cycle and improve the streptomycete production efficiency, the microbial inoculum has high cellulose activity, and can effectively degrade fruit branches, straw, cassava dregs, and the like, a biological organic fertilizer is prepared, and considerable economic benefits are created. 本发明涉及种植物生长调节剂应用于链霉菌菌剂制备的方法，包括如下步骤：1)菌种摇床培养；2)种子液制备；3)发酵液制备；4)菌剂的固定。本发明采用三级发酵方式实