Identification method of ST37 type clostridium difficile and unit point variation type ST81 thereof

• Main Authors: ZHAI YU, QIANG CUIXIN, SHI DONGYAN, LI ZHIRONG, ZHAO BAOXIN, YANG JING, LI RUXIN, SUN SUJU, WEI HONGLIAN, ZHAO FENG, WANG XIAOMING, ZHAO JIANHONG, ZHAO XINGZHEN
• Format: Patent
• Language: Chinese; English
• Published: 26.09.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an identification method of ST37 type clostridium difficile and unit point variation type ST81 thereof. The identification method comprises the following steps: protein in a clostridium difficile strain to be identified is extracted to obtain a mass spectrometric text sample; and mass spectrometric detection equipment is utilized to detect the sample, and strains with specific protein peaks at m/z 3235-3245 and m/z 3280-3290 in an obtained mass spectrogram are the ST37 type clostridium difficile or the unit point variation type ST81 thereof. The invention establishes an MALDI-TOF MS-based rapid identification method of the ST37 type clostridium difficile and the unit point variation type ST81 thereof for the first time. The method has the characteristics of real time and rapidness, can screen the ST37 type clostridium difficile and the unit point variation type ST81 thereof in real time while identifying the strains, only needs to add 1min for the detection process of ST37 or ST81 type