Fluorescent dye molecule with biological polarity sensitivity and preparation method thereof

• Main Authors: QI BUYUN, CHEN DUGANG, YU XIANGLIN, LI JUNBO
• Format: Patent
• Language: Chinese; English
• Published: 22.09.2017
• Subjects: ADHESIVES; CHEMISTRY; DYES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LAKES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; MORDANTS; NATURAL RESINS; ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; PAINTS; PHYSICS; POLISHES; TESTING

The invention discloses a fluorescent dye molecule with biological polarity sensitivity and a preparation method thereof. The fluorescent dye molecule provided by the invention is shown by the formula I in the specification. The compound is a D-pi-A-pi-D type conjugated compound using aryl nitrogen as a donor (D) and using alpha-beta-unsaturated ketone as an acceptor (A). The acceptor unit has the tautomeric equilibrium structure of the enol form and the diketone form in different polarity environments (shown as the formula III), so that the system conjugation degree change is caused; the compound luminescent property change is caused, so that the formula I has good polarity sensitivity. The fluorescent dye molecule provided by the invention has great fluorescent property differences in different polar solvents; the cell imaging experiments show that the obvious biological polarity sensitivity is realized; the dynamic change of the polarity in the cells can be tracked on the basis. 本发明公开了具有生物极性敏感性的荧光染料分子及其制备方