基于对比染色的深部真菌形态学快速检测方法及试剂

• Format: Patent
• Language: Chinese
• Published: 15.05.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

本发明公开了一种基于对比染色的深部真菌形态学快速检测方法，涉及一种用于检测深部真菌的方法。它包括对深部真菌检测所需的器具进行无菌消毒和保存方法及无菌操作方法；取被检患者肘静脉血约1ml，在无菌环境中将一滴未凝固的静脉血滴入盛有0.8ml，1％芝加哥天蓝生理盐水溶液的安瓿瓶中稀释15倍并染色，在无菌条件下将一滴经过染色的混合液滴于无菌的载玻片上，盖上盖玻片后工作人员通过显微镜和500万像索以上的电子目镜放大后，用高分辨率显像软件将载玻片上的混合液的图像显示于电脑屏幕进行观察记录。本发明能清晰显示各种活体细胞，可为临床疾病的检测带来革命性的改变。本发明还涉及一种基于对比染色的深部真菌形态学快速检测试剂。 A method of detecting invasive fungi according to morphology thereof based on contrast staining, including: sterilizing and storing the necessary equipment aseptically; drawing 1 ml of venous blood from a tested subject's elbow vein; dripping one drop of the venous blood, prior to coagulation, into an ampoule containing 0.8 ml of a detection reagent under an aseptic environment; gently shaking the ampoule until the drop of venous blood is evenly distributed; leaving the ampoule to stand for 20 minutes to form a stained solution; sterilizing or disinfecting a microscope slide and a cover slip; dripping one drop of the stained solution on the microscope slide prepared under aseptic condition; observing the sample