Detection kit applicable for controlling gait of amble of Yanqi horse and application

• Main Authors: CAI BATE, DENG HAIFENG, LI HAI, AIERDENGBAYIER, QI ALADAER
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a detection kit applicable for controlling a gait of amble of Yanqi horse and application. Specific primers are designed according to a gene sequence of a Yanqi horse DMRT3, the positive primer is 5'-AGCGACAAAGACACCGACCAG-3', and the negative primer is 5'-TTGCCAAAGTATTCCTCAGCATCA-3'; by taking a sample genome DNA as a template, PCR (Polymerase Chain Reaction) amplification is carried out, and electrophoresis analysis is carried out after enzyme digestion on a product subjected to PCR amplification is carried out, and if double stripes appear, and Yanqi horse are determined as the Yanqi horse having the gait of amble; if single stripe or three stripes appear, Yanqi horse are determined as the Yanqi horse having the gait of non-amble, and Yanqi horse having the gait of amble can be judged and picked by utilizing the difference of electrophoretic stripes. According to the detection kit disclosed by the invention, a detection method of the Yanqi horse having the gait of amble is short in d