Subject anti-hcv antibody detection assays employing ns3 capture peptides

• Main Authors: PROSTKO JOHN, MUERHOFF ANTHONY S, MAROHNIC CHRIS
• Format: Patent
• Language: Chinese; English
• Published: 29.08.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a 'double shot' of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing). 本发明提供了使用NS3捕获肽用于检测样品中的受试者抗HCV抗体的方法、试剂盒和组合物。在某些实施方案中，起采用了至少两种NS3解旋酶（NS3h）捕获肽和至少两种缀合肽（例如NS3h缀合肽），其允许在步式测定中的受试者抗体检测的宽动态范围。在其他实施方案中，提供了在不使用还原剂的情况下检测NS3特异性受试者抗体的方法。在些实施方案中，NS3特异性受试者抗体用NS3缀合肽'双击'进行检测（例如，在洗涤之前和之后均添加缀合肽至样品中）。