Primer combination and kit for simultaneous detection of 93 bovine genetic defect genes and lethal haplotypes
The invention discloses a primer combination and a kit for simultaneous detection of 93 bovine genetic defect genes and lethal haplotypes. Through use of the primer combination and the kit, casual mutation sites (SNP and insertion or deletion of short fragments) of the 93 genetic defect genes (such...
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Main Authors | , , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
29.08.2017
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a primer combination and a kit for simultaneous detection of 93 bovine genetic defect genes and lethal haplotypes. Through use of the primer combination and the kit, casual mutation sites (SNP and insertion or deletion of short fragments) of the 93 genetic defect genes (such as PIRM syndrome, crooked tail syndrome, epidermolysis bullosa and the like) and the lethal haplotypes (HH1, HH3, HH4, HH5 and JH1) can be detected in one time, cattle with monogenic inheritance defect gene carriers and lethal haplotype carriers can be screened, and accurate guidance is provided for cattle breeding and genetic evaluation, herd selection and assortative mating, population genetic improvement, cultivation of new varieties and genetic resource protection. The primer combination and the kit have the characteristics of high throughput, low cost, high accuracy, and the like, is suitable for the varieties of beef cattle and dairy cattle, and can be widely used in the breeding and reproduction field of the |
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Bibliography: | Application Number: CN201710438948 |