Tetracycline detection method and detection kit

The invention discloses a tetracycline detection method and a detection kit. The tetracycline aptamer is adopted as a molecular recognition element, for the design of non-stem-loop structure nucleic acids, when tetracycline is present in the system, a DNA self-assembly can be started, and the amplif...

Full description

Saved in:
Bibliographic Details
Main Authors CHEN JUNHUA, LI DINGQIANG
Format Patent
LanguageChinese
English
Published 25.08.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention discloses a tetracycline detection method and a detection kit. The tetracycline aptamer is adopted as a molecular recognition element, for the design of non-stem-loop structure nucleic acids, when tetracycline is present in the system, a DNA self-assembly can be started, and the amplification of detection signals is achieved, and a large number of G-rich base nucleic acid sequences are substituted out. The finally formed G tetramer has horseradish peroxidase-like catalytic activity, the TMB-H2O2 detection system can be catalytically oxidized, the colorless substrate is turned into blue in color, the concentration of tetracycline and changes in blue color are positively correlated, and the concentration of tetracycline in the detection system can thus be judged. The tetracycline detection method is high in sensitivity, the detection limit of tetracycline is 10 pM, and the detection is excellent in specificity, common interferences do not have an impact on detection. Detection instruments are not
Bibliography:Application Number: CN20171147202