Synthesis of DNA

• Main Authors: ROTHWELL PAUL, EXTANCE JONATHAN, PORTER NEIL
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to synthesis of DNA. The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner. 本发明涉及用于合成DNA、RNA、蛋白质及类似分子的改进的方法，尤其是DNA的无细胞酶促合成，优选以大的规模。本发明涉及使用链置换复制的DNA合成，且控制向反应混合物添加核苷酸，从而控制产率。反应混合物含有起始量的核苷酸、聚合酶和DNA模板，以受控的方式向其供给另外的核苷酸。