Synthesis of DNA

The invention relates to synthesis of DNA. The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-di...

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Bibliographic Details
Main Authors ROTHWELL PAUL, EXTANCE JONATHAN, PORTER NEIL
Format Patent
LanguageChinese
English
Published 18.08.2017
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Summary:The invention relates to synthesis of DNA. The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner. 本发明涉及用于合成DNA、RNA、蛋白质及类似分子的改进的方法,尤其是DNA的无细胞酶促合成,优选以大的规模。本发明涉及使用链置换复制的DNA合成,且控制向反应混合物添加核苷酸,从而控制产率。反应混合物含有起始量的核苷酸、聚合酶和DNA模板,以受控的方式向其供给另外的核苷酸。
Bibliography:Application Number: CN201580047669