Detection probes, gene chip and method for Rhodotorula glutinis in dairy products

• Main Authors: FAN XIAOJUN, LIU JIAN, LI YAO, LI MIN, NIE FANYU
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a detection method for Rhodotorula glutinis in dairy products. The detection method comprises the following steps: (1) target sequence determination; (2) primer and probe design; (3) template extraction; (4) RT-PCR amplification and fluorescence labeling; (5) chip preparation; (6) molecular hybridization; (7) result analysis. By means of the gene chip, Rhodotorula glutinis can be detected qualitatively and quantitatively within short time, the method is applicable to detection of the dairy products of milk source samples, produced intermediate goods, finished products and the like on whole process chains, and one novel detection method with quality control in a production process can be provided while the shelf life of the dairy products is shortened substantially. 本发明公开了种乳制品中粘红酵母的检测方法，包括如下步骤：（1）、靶序列的确定；（2）、引物与探针的设计；（3）、模板提取：（4）、RT-PCR扩增及荧光标记；（5）、芯片制备；（6）、分子杂交；（7）、结果分析。本发明通过该基因芯片能够在短时间内粘红酵母Rhodotorula glutinis进行定性定量检测，本发明方法适用于奶源样品、生产中间品、成品等全工艺链上的乳品检测，在大幅缩短乳品货架期同时还可以提供种生产过程质控的新型检测方法。