Method for obtaining salt-tolerant transgenic soybean material

• Main Authors: MA HONG, ZHANG LING, ZHANG YUANYU, LI FEIWU, TAN XICHANG, DONG YINGSHAN
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of plant genetic engineering and provides a method for obtaining a salt-tolerant soybean new material by utilizing a transgenic technology. The method comprises the following steps: (1) introducing a plant expression vector pYL-AgGlpF containing a gene AgGlpF into a receptor soybean genome by utilizing agrobacterium tumefaciens-mediated soybean cotyledonary node genetic transformation method; and (2) carrying out molecular screening and salt tolerance identification, so that a transgene AgGlpF salt-tolerant soybean material is obtained. The method provided by the invention has the advantages that a gene AgGlpF is transferred into the soybean genome, and salt tolerance of soybeans can be obviously improved, so that the method provided by the invention has important economic values and broad application prospects on agricultural production. 本发明属于植物基因工程技术领域，提供了种利用转基因技术获得耐盐大豆新材料的方法，该方法包括以下步骤：（1）利用根癌农杆菌介导的大豆子叶节遗传转化法，将含有基因的植物表达载体pYL-AgGlpF导入受体大豆基因组中；（2）通过分子筛选和耐盐性鉴定，获得转基因