Genetically engineered bacterium for synthesizing 2,3-butanediol by co-catalysis of isozyme and construction method and application thereof

• Main Authors: LIU DONG, YING HANJIE, SHEN XIAONING, CHEN YONG, ZHUANG WEI, YANG ZHENGJIAO, ZHU CHENJIE, WU JINGLAN
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method of synthesizing 2,3-butanediol by co-catalysis of isozyme. By means of co-catalysis of more than two 2,3-butanediol dehydrated proteins (also called as 2,3-butanediol dehydrogenase) with a complementary catalytic advantage, a precursor of 2,3-butanediol is efficiently and thoroughly converted into 2,3-butanediol. The method of synthesizing 2,3-butanediol by co-catalysis provided by the invention not only can increase the efficiency of synthesizing 2,3-butanediol, but also can fully convert the precursor substance thereof, so that the flux of a 2,3-butanediol synthetic branch in a metabolic network and the economical benefit of the 2,3-butanediol synthetic process can be remarkably improved. 本发明公开了种利用同工酶共催化合成2,3-丁二醇的方法，通过两个以上具有互补性催化优势的2,3-丁二醇脱氢蛋白(又称2,3-丁二醇脱氢酶)的共同催化，能够将2,3-丁二醇的前体高效并且彻底地转化为2,3-丁二醇。本发明通过同工酶共催化合成2,3-丁二醇的方法，不仅能够提高2,3-丁二醇合成的效率，还能够实现其前体物质完全转化，因而能够显著提高细胞代谢网络中2,3-丁二醇合成支路的通量以及2,3-丁二醇合成过程的经济性。