Detection kit for CYP2C19 and ABCB1 genes

• Main Authors: LIN YAN, ZHONG YU, WANG YUHAN, LI QING, MA QINGWEI, ZHANG HAIYAN, YANG YUFENG, WU NALAHU
• Format: Patent
• Language: Chinese; English
• Published: 11.08.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a primer combination for detecting gene polymorphic sites relevant to clopidogrel resistance, wherein the sites comprise the polymorphic sites including CYP2C19*2, *3, *4, *5, *17 and the polymorphic site 3435C>T of ABCB1. According to the scheme, multiple PCR primers are utilized for amplifying gene segments where relevant gene loci are located, after the amplification product is treated, single base extension is carried out on the to-be-detected loci, then molecular weight difference detection is carried out on the extension product by adopting a flight time mass spectrum, through data analysis, the result of the relevant gene for the clopidogrel resistance of a patient is rapidly detected. The invention further provides a detection product prepared by adopting the primer combination and applications of the detection product, and thus references are provided for the individualized medication of clopidogrel. 本发明提供种检测与氯吡格雷抵抗相关的基因多态位点的引物组合物，其中所述位点包括CYP2C19*2、*3、*4、*5、*17以及ABCB1的3435C>T多态