High-polymer adsorbing material for separating and purifying vitamin B12 from fermentation liquid

• Main Authors: XU JIANZHONG, SUN KAI, CHAO YUEGANG, GUI JINQIU, LIANG JUN, CAI KERUI, YAN LEI, HU BINGJIAO, SONG TIEJUN, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 11.08.2017
• Subjects: AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; GENERAL PROCESSES OF COMPOUNDING; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; METALLURGY; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SUGARS; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING; WORKING-UP

The invention discloses a high-polymer adsorbing material for separating and purifying a vitamin B12 from fermentation liquid, and relates to the technical field of biological medicine extraction. A preparing method of the high-polymer adsorbing material includes the following steps of (1) macroporous adsorption resin preparing; (2) macroporous adsorption resin pretreating; (3) macroporous adsorption resin modifying. The vitamin-B12 adsorbing capacity of the macroporous adsorption resin prepared with the method is 18.5 mg/g or above, efficient desorbing of the vitamin B12 can be achieved after washing is carried out through a 65% methanol solution, the desorption rate is 98.5% or above, the purity of the prepared vitamin B12 is 58% or above, and the macroporous adsorption resin is suitable for separating and purifying the vitamin B12 from the fermentation liquid accordingly. 本发明公开了种用于从发酵液中分离纯化维生素B的高分子吸附材料，涉及生物医药提取技术领域，其制备方法包括如下步骤：(1)大孔吸附树脂的制备；(2)大孔吸附树脂的预处理；(3)大孔吸附树脂的改性。本发明所制大孔吸附树脂对维生素B的吸附量达到18.5mg/g以上，经65％甲醇溶