Application of nitrogen nutrition transport gene OsNPF8.1 in improvement of rice tiller number

• Main Authors: WANG JIE, NIE HAIPENG, LYU KAI, FANG ZHONGMING, HUANG WEITING
• Format: Patent
• Language: Chinese; English
• Published: 04.08.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses application of a nitrogen nutrition transport gene OsNPF8.1 in improvement of rice tiller number and belongs to the field of plant genetic engineering. An OsNPF8.1 gene encoded protein has an amino acid sequence shown as SEQ ID NO.1, and a cDNA sequence of the protein is shown as SEQ ID NO.2. By establishing rice OsNPF8.1 gene overexpression plants and OsNPF8.1 gene interference plants, the tiller number of normal rice and the spike number of each rice plant can be increased by improving the OsNPF8.1 gene expression. Therefore, the gene OsNPF8.1 can be used in rice breeding for improving the rice yield. The gene OsNPF8.1 has important application values in the aspect of explaining influences of nitrogen on plant growth and development and the aspect of improving the rice plant type. 本发明公开了氮营养运输基因在提高水稻分蘖数中的应用，属于植物基因工程领域。基因编码蛋白质的氨基酸序列如SEQ ID NO.1所示，cDNA序列如SEQ ID NO.2所示。本发明通过构建水稻基因超表达植株、基因干扰植株，发现通过提高基因表达，可以使正常的水稻分蘖数和每株穗数增加，因此基因可用于水稻选育中以提高水稻产量。基因在阐述氮素影响植物生长及发育过程方面以及在水稻株型改良方面具有重要的应用价值。