DNA batch obtaining method applying in detecting purity of oilseed rape hybrids by molecular markers

• Main Authors: ZHAO KAIQIN, CHEN WEI, FU MINGLIAN, LUO YANQING, ZU FENG, LI GENZE, WANG JINGQIAO, LI XIA
• Format: Patent
• Language: Chinese; English
• Published: 01.08.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERTILISING; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PLANTING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SOWING; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a DNA batch obtaining method applying in detecting purity of oilseed rape hybrids by molecular markers. Seamless joint in seed germination and DNA extraction process is achieved through a method of oilseed rape hybrid germination in deep pore plates, the efficiency of obtaining DNA is improved, manpower and material resources are saved, and experimental costs are compressed. On the basis of the DNA batch obtaining method applying in detecting the purity of the oilseed rape hybrids by the molecular markers, extraction steps of DNA by an alkaline lysis method are further simplified, and an idea of completing extraction of ten thousand DNA samples within 10 hours is achieved by coordinating with the use of high-throughput triturator Fast & Fluid Management sample mixers. The DNA batch obtaining method applying in detecting the purity of the oilseed rape hybrids by the molecular markers is a whole set of intensification scheme obtaining oilseed rape cotyledon DNA samples in rapid speed, ba