DNA batch obtaining method applying in detecting purity of oilseed rape hybrids by molecular markers
The invention discloses a DNA batch obtaining method applying in detecting purity of oilseed rape hybrids by molecular markers. Seamless joint in seed germination and DNA extraction process is achieved through a method of oilseed rape hybrid germination in deep pore plates, the efficiency of obtaini...
Saved in:
Main Authors | , , , , , , , |
---|---|
Format | Patent |
Language | Chinese English |
Published |
01.08.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The invention discloses a DNA batch obtaining method applying in detecting purity of oilseed rape hybrids by molecular markers. Seamless joint in seed germination and DNA extraction process is achieved through a method of oilseed rape hybrid germination in deep pore plates, the efficiency of obtaining DNA is improved, manpower and material resources are saved, and experimental costs are compressed. On the basis of the DNA batch obtaining method applying in detecting the purity of the oilseed rape hybrids by the molecular markers, extraction steps of DNA by an alkaline lysis method are further simplified, and an idea of completing extraction of ten thousand DNA samples within 10 hours is achieved by coordinating with the use of high-throughput triturator Fast & Fluid Management sample mixers. The DNA batch obtaining method applying in detecting the purity of the oilseed rape hybrids by the molecular markers is a whole set of intensification scheme obtaining oilseed rape cotyledon DNA samples in rapid speed, ba |
---|---|
Bibliography: | Application Number: CN20171111484 |