Novel selectable marker genes

• Main Authors: WRIGHT TERRY R, ARNOLD NICOLE L, WEBB STEVEN ROBERT, ROBINSON ANDREW E, LIRA JUSTIN M, RUSSELL SEAN M, MERLO DONALD J, SMITH KELLEY A
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The subject invention relates to novel selectable marker genes, specifically to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention. 本发明涉及新型选择性标记基因，具体涉及在本文称作DSM-2的新基因。这种基因是在天蓝色链霉菌A3中鉴定的。DSM-2蛋白与PAT和BAR的关系较远。主题的发明还提供按植物优化的编码DSM-2蛋白的基因。DSM-2可以用作转基因性状以在植物和植物细胞中赋予对除草剂草铵膦和双丙氨酰膦的耐受。主题的基因的种优选用途是作为选择性标记。在