A hollow nanosphere used for loading lipase for vanillin preparation through catalytic oxidation

• Main Authors: HAN YANLONG, CUI RONGJUN, LIU CHUNLING, SUN KAI, XU HONGNA, GUI JINQIU, CHAO YUEGANG, CAI KERUI, YAN LEI, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 14.07.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

A hollow nanosphere used for loading lipase for vanillin preparation through catalytic oxidation is disclosed and relates to the technical field of biological catalysis. The method includes (1) preparing a polymer hollow nanosphere; (2) preparing a polymer-TiO2 composite hollow nanosphere; (3) covering the composite hollow nanosphere with a hard shell; and (4) preparing the disclosed hollow nanosphere. The prepared hollow nanosphere is used for loading the lipase. The lipase loading amount in each gram of the hollow nanosphere is 15 mg or above. A prepared lipase catalyst is specially used for vanillin preparation through catalytic oxidation, coniferyl aldehyde is adopted as a raw material, hydrogen peroxide-urea is adopted as an oxygen source of the lipase, the yield of prepared vanillin is 93% or above, and purity of the prepared vanillin is 98% or above. 本发明公开了种通过催化氧化法制备香兰素的脂肪酶负载用空心纳米球，涉及生物催化技术领域，其制备方法包括如下步骤：(1)聚合物空心纳米球的制备，(2)聚合物-TiO复合空心纳米球的制备，(3)复合空心纳米球表面硬质外壳的包覆，(4)空心纳米球的制备。本发明所制空心纳米球用于负载脂肪酶，每克空心纳米球上脂肪酶的负