Preparation method for reprogramming somatic cells into neural stem cells by microRNA, and application of neural stem cells

• Main Author: WANG YUESI
• Format: Patent
• Language: Chinese; English
• Published: 11.07.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a method for induced transdifferentiation of somatic cells into neural stem cells, and an application of the neural stem cells, and concretely relates to neural stem cells with high yield, good pluripotency and good passage stability, formed through inducing human fibroblasts, epithelial cells, blood cells, adipocytes and other somatic cells in a normal physiological environment via a reprogramming technology by using microRNA-302, valproic acid, polybrene, epidermal growth factor and fibroblast growth factor. The method utilizes single microRNA to transfer the somatic cells without introducing an exogenous transcription factor, the clone forming time in the invention is much shorter than that in the prior art, and the neural stem cells are hopeful for developing therapeutic methods for drugs for treating degenerative neurological diseases (including spinal cord injury and other lesions), so the neural stem cells have a good clinical application prospect. 本发明提供了种诱导体细胞转分化为神经干细胞的方法及其应用。具体