Method for separating and purifying in-vivo metabolites of protopanaxadiol

• Main Authors: LONG JIAKUN, LING JIN, DUAN GENGLI, YU YINGJIA
• Format: Patent
• Language: Chinese; English
• Published: 04.07.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for separating and purifying in-vivo metabolites of protopanaxadiol in excrement, belonging to the field of pharmacoanalysis. The method employs a silica-gel chromatographic column for crude separation and then employs mass spectrometry-based preparative liquid chromatography for purification; and through combination of the above two separation methods, pure metabolites are prepared. According to the method, separation via the silica-gel chromatographic column and separation via preparative liquid chromatography are successively applied so as to rapidly and efficiently purify a plurality of pure metabolites in one shot; gradient elution conditions of the silica-gel chromatographic column and the preparative liquid chromatography enable a plurality of unknown substances to be separated and purified in a short period of time; the method uses excrement us a to-be-separated sample, and the to-be-separated sample is low in price, easy to acquire and accumulate and convenient for s